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Filter Combinations for Epi-Fluorescence Recommendation by Carl Zeiss

Fluorochrome: [ A-C ] [ D-G ] [ H-P ] [ Q-Z ]

Fluorochrome	Spectral Data		Filter sets
	Exitat.	Emiss.	recommended filter combination	combination suitable to a limit extent	filter set for synchronous double/tripple fluorescence
QUIN 2	340/ 365	490		1,2
Quinacrine mustard	440	510	5,6
Resorufin	571	585	14,15
Rhodamine	540- 560	580	14,15
Rhodamine 123	540- 560	580	15
Rhodamin phalloidin	550	575	14,15
Rhodol green	500	525		17
Acid fuchsine	540	630	0,14,15
SBFI	340/ 380	420	21,30
Serotonin	365	520- 540	1,2
SNARF	480	600/ 650	on requestx)
Stilbene SITS, SITA	365	460	1,2
Sulfaflavin	380- 470	470- 580	1,2
Tetracycline	390	560	5,18	7
Tetrametylrhodamin	540	566	14,15
Texas red	595	620	0		24,25
Thioflavine S	430	550	5,6,7
Thiazin red	510	580	14
TOTO-1 + DNA	509	533	9,10	14,15
TOTO-3 + DNA	642	661	26
TRITC	540	580	14,15		23,28
XRITC	560	620	0,14,15		23
YOYO-1	491	509	9,10
YOYO-3	642	660		26
Xylenol orange	377	610	14,15
Immunogold, Silber	-	-	H/Pol

legend: x) combination on request (partly compiled from non-standard filters). All filter combinations are mentioned for Axio-line microscopes. We like to inform you about the new double and tripple bandpass filter sets. At the same time we present a new list of fluorochromes for our filter combinations. Please take this as an invitation to communicate about important missing fluorochromes or fluorochromes that nobody uses any longer. Please remind that shown maxima of excitation and emmision don´t fit to every application and therefore the indicated filter set must´nt be at optimum. If the operator uses another salt of the compound or the pH-value is different than excitation and emission could be changed completely. In such cases we will help you: [email protected]. Filter sets:               Filter set 0                    Filter set 1                 Filter set 2                 Filter set 5               Filter set 6                    Filter set 9                 Filter set 10               Filter set 14               Filter set 15                  Filter set 16               Filter set 17               Filter set 18               Filter set 21/30             Filter set 23               Filter set 24               Filter set 25               Filter set 26                  Filter set 27               Filter set 28

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