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Filter Combinations for Epi-Fluorescence Recommendation by Carl Zeiss

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Fluorochrome	Spectral Data		Filter sets
	Exitat.	Emiss.	recommended filter combination	combination suitable to a limit extent	filter set for synchronous double/tripple fluorescence
Dansylamide	340	578	1,2
Dansylchloride	380	475	1,2
DAO			1,2
DAPI + DNA	359	461	1,2		25,27,28
DASPMI			5
DiBAC4 Dibutylbarbituric acid trimethine oxonol	439	516	6,7
DIDS thiocyanato- stilbene	342	418	1,2
DiIC3/4/12/22 indocarbocyanine	540- 560	556- 575	14,15	0,26
DiOC2/7/16 carbocyanine	550	580	15	0,14
DiSC1/2/3/6 thiacarbocyanine	559	585	14,15	26
Diaminonaphtylsulfanic acid	340	525	1,2
Dopamin	340	490- 520	5
DTAF	495	528	9,10
Ethidium bromide + DNA	510	595	14,15
Euchrysin	430	540	5,6,7
Evans blue	550	611	14,15
Fastblue			18	2,5
Feulgen	480	560	14,15
FIF - Falk	405	435	18
Fluo-3	480	520	9,10
FLURAM Fluorescamine			1,2
FDA Fluoresceindiacetate	499		9
FITC Fluorescein- isothiocyanate	490	525	9,10,16,17		23,24,25,27
Fluorogold	350- 395	530- 600	on requestx)
Blue FluoSpheres	360	415	1,2
Crimson FluoSpheres	625	645	0
Red FluoSpheres	580	605	0,14
Dark Red FluoSpheres	650	690	26
Yellow-Green FluoSpheres	490	515	9,10
FURA-2	340/ 380	500/ 530	21,30
Generic blue/green combination			9,10
GFP blue (Green Fluoresc. Protein)	382	448	1,2
GFP green	471	503	9,10,16,17
GFP Mutant W	458	480	not yet recommendation!
GFP Wild Type	396	508	5,18

legend: x) combination on request (partly compiled from non-standard filters). All filter combinations are mentioned for Axio-line microscopes. We like to inform you about the new double and tripple bandpass filter sets. At the same time we present a new list of fluorochromes for our filter combinations. Please take this as an invitation to communicate about important missing fluorochromes or fluorochromes that nobody uses any longer. Please remind that shown maxima of excitation and emmision don´t fit to every application and therefore the indicated filter set must´nt be at optimum. If the operator uses another salt of the compound or the pH-value is different than excitation and emission could be changed completely. In such cases we will help you: [email protected]. Filter sets:               Filter set 0                    Filter set 1                 Filter set 2                 Filter set 5               Filter set 6                    Filter set 9                 Filter set 10               Filter set 14               Filter set 15                  Filter set 16               Filter set 17               Filter set 18               Filter set 21/30             Filter set 23               Filter set 24               Filter set 25               Filter set 26                  Filter set 27               Filter set 28

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