(Fluorescence-In-Situ-Hybridization)

Fluorescence in situ hybridization allows the direct localization of DNA and RNA sequences on chromosomes, in cells and in tissue. This technology is based on the hybridization between target sequences of the single-strand DNS of chromosomes or cell nuclei with labeled complementary specimens. The signal is intensified by means of specific fluorochrome-labeled antibodies and visualized in the microscope.

This technique allows, for example, the localization of genes and also the direct morphological detection of genetic defects causing hereditary diseases.

Other applications:

• detection of diagnostically significant aneuploids, e.g. trisomy 21
• chromosome painting
• interphase cytogenetics
• CGH method (Comparative Genomic Hybridization)
• Prenatal diagnostics

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